Description
Custom synthesis for His-tag (polyhistidine) PEGylation reagent is available for PEG-NTA. It includes mPEG-NTA, NTA-PEG-NTA, 4/8-Arm PEG-NTA at various MW. Nitrilotriacetic acid (NTA)-PEG conjugate in the form of a metal complex with Co colbat, Ni Nickel form a chelating complex with polyhistidine tag. A polyhistidine-tag consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. His-tag synonyms: hexa histidine-tag, 6xHis-tag, His6 tag. His-tag is a registered Trademark of EMD Biosciences. His-tag is common to tag recombinant proteins for easy purification. mPEG-NTA can effectively PEGylate polyhistidine tagged proteins. Contact us.
Properties
Custom synthesis is available for PEG-NTA including:
mPEG-NTA MW 350, 550, 750, 1k, 2k, 5k, 10k, 20k, 30k and 40k
NTA-PEG-NTA MW 600, 1k, 2k, 3.4k, 5k, 10k, 20k and 30k
4-arm PEG-NTA MW 2k, 5k, 10k and 20k
8-arm PEG-NTA MW 10k, 20k and 40k
Maleimide-PEG-NTA MW 1k, 2k, 3.4k, 5k and 10k
Acrylate-PEG-NTA MW 1k, 2k, 3.4k, 5k and 10k
Biotin-PEG-NTA MW 1k, 2k, 3.4k, 5k and 10k
FITC-PEG-NTA MW 1k, 2k, 3.4k, 5k and 10k
and many others
References
His-tag and Ni-NTA (Nickel-Nitrilotriacetic acid) are fundamental components for recombinant protein purification, relying on the strong, specific binding between a short string of histidine residues (the His-tag) and immobilized nickel ions on a resin (Ni-NTA). This technique, called Immobilized Metal Affinity Chromatography (IMAC), uses Ni-NTA’s high affinity for the tag to capture target proteins from complex cell lysates, allowing easy purification under native or denaturing conditions for downstream applications like detection or functional studies.Â
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