Description
OPSS-PEG-NHS is a linear heterobifunctional PEGylation reagent with an OPSS and a succinimidyl NHS ester. It is a useful crosslinking reagent with a PEG spacer. OPSS stands for ortho-pyridyl disulfide or ortho pyridyldithio, PDP, pyridyl dithio propionate. OPSS reacts with thiol, SH, sulfhydryl or mercapto to form a S-S disulfide bond. Under certain conditions, OPSS produces free thiol for direct PEGylation of noble metal such as gold and silver nanoparticles. The NHS ester is succinimidyl carboxyl methyl ester (SCM), highly reactive toward amine.
Properties
Molecular weight: MW of PEG was measured by MALDI-MS or GPC. PDI (polydispersity index) of our linear PEG is 1.02-1.05 with very narrow MW distribution. The number of repeating ethylene oxide units (CH2CH2O) or the degree of polymerization is calculated dividing the PEG MW by 44 (44 is the molecular mass of one repeating unit).
Solubility: Soluble in water and aqueous buffer, chloroform, methylene chloride, DMF, DMSO, and less soluble in alcohol, toluene. Not soluble in ether.
Density: PEG density is approximately 1.125 g/mL
Physical form: PEG products generally appear as white or off-white powder, and for very low MW linear PEG such as MW 1k or less, it may appear as wax-like, semi-solid material due to the low MW and the type of functional groups.
Storage condition: PEG product shall be stored in the original form as received in a freezer at -20C or lower for long term storage. Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. Stock solution should avoid repeated freeze-and-thaw cycles. See Documents section for detailed storage and handling conditions.
References
1. Silica-gold nanoshells as potential intraoperative molecular probes for HER2-overexpression in ex vivo breast tissue using near-infrared reflectance confocal microscopy, Breast Cancer Res Treat, 2010; 120(3):547-55, Text.
2. Evaluation of Immunotargeted Gold Nanoshells as Rapid Diagnostic Imaging Agents for HER2-Overexpressing Breast Cancer Cells: A Time-based Analysis, Nanobiotechnology, pp. 1-8, 2008, Text.
3. Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy, Nanotechnology 19 (2008) 315102, Text.
4. Detection of chronic lymphocytic leukemia cell surface markers using surface enhanced Raman scattering gold nanoparticles, Cancer Lett. 2010, 1;292(1):91-7, Text.
5. Biological evaluation of RGDfK-gold nanorod conjugates for prostate cancer treatment. Journal of Drug Targeting, 2011; 19(10): 915–924, Text.
6. Controlled release of Rituximab from gold nanoparticles for phototherapy of malignant cells, Journal of Controlled Release, 162 (2012) 303-309, Text.
7. Gold nanorods for target selective SPECT/CT imaging and photothermal therapy in vivo. Quant Imaging Med Surg, 2012;2:1-11, Text,
8. Gold nanostars: surfactant-free synthesis, 3D modelling, and two-photon photoluminescence imaging, Nanotechnology. 2012; 23(7): 075102, Text,
9. Multiplexed Dot Immunoassay Using Ag Nanocubes, Au/Ag Alloy Nanoparticles, and Au/Ag Nanocages, Nano Res. 2012, 5(2): 124-134, Text,
10. Targeted Delivery of siRNA to Hepatocytes and Hepatic Stellate Cells by Bioconjugation, Bioconjugate Chem. 2010, 21, 2119-2127, Text.
11. Au@Pt Core-Shell Nanoparticle Bioconjugates for the Therapy of HER2+ Breast Cancer and Hepatocellular Carcinoma. Model Studies on the Applicability of 193mPt and 195mPt Radionuclides in Auger Electron Therapy, Molecules 2021, 26, 2051, molecules-26-02051.
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