Description
OPSS-PEG-COOH is a linear heterobifunctional PEG reagent with an OPSS and a carboxyl group. It is a useful crosslinking reagent with a PEG spacer. OPSS stands for orthopyridyl disulfide or ortho pyridyldithio, or PDP, pyridyl dithio propionate. OPSS reacts with thiol, SH, sulfhydryl or mercapto to form a S-S bond. The COOH can react with amine or hydroxyl under standard acid/amine or acid/alcohol coupling conditions. OPSS under certain conditions generates free thiol for direct PEGylation with novel metal such as gold and silver nanoparticles.
Properties
Molecular weight: MW of PEG was measured by MALDI-MS or GPC. PDI (polydispersity index) of our linear PEG is 1.02-1.05 with very narrow MW distribution. The number of repeating ethylene oxide units (CH2CH2O) or the degree of polymerization is calculated dividing the PEG MW by 44 (44 is the molecular mass of one repeating unit).
Solubility: Soluble in water and aqueous buffer, chloroform, methylene chloride, DMF, DMSO, and less soluble in alcohol, toluene. Not soluble in ether.
Density: PEG density is approximately 1.125 g/mL
Physical form: PEG products generally appear as white or off-white powder, and for very low MW linear PEG such as MW 1k or less, it may appear as wax-like, semi-solid material due to the low MW and the type of functional groups.
Storage condition: PEG product shall be stored in the original form as received in a freezer at -20C or lower for long term storage. Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. Stock solution should avoid repeated freeze-and-thaw cycles. See Documents section for detailed storage and handling conditions.
References
1. Quantitative and multiplex dot-immunoassay using gap-enhanced Raman tags, RSC Adv., 2017, 7, 40834-40841, Text.
2. Gold nanoparticle-aided preparation of antibodies to ÃŽ ±-methylacyl-CoA racemase and its immunochemical detection, Gold Bull (2016) 49: 87. Text.
3. Targeting CD4+ Cells with Anti-CD4 Conjugated Mertansine Loaded Nanogels, Biomacromolecules, 2020, DOI: 10.1021/acs.biomac.0c00442
4. Antibody-Gold Nanoparticle Bioconjugates for Biosensors: Synthesis, Characterization and Selected Applications, Biosensors and Bioelectronics, 2020, 112370, doi: 10.1016/j.bios.2020.112370
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