mPEG-Cholesterol uses very stable chemical bonds/linkage to attach cholesterol to PEG through ether bond. Unlike ester bonds which are prone to hydrolysis in aqueous solution, the ether linkage between cholesterol and PEG is chemically robust and very stable. Cholesterol is a membrane constituent which serves a unique function of modulating membrane fluidity, elasticity, and permeability. It fills in the gaps created by packing of other lipid species when proteins are embedded in the membrane. PEGylated cholesterol as an amphiphilic lipid derivative can be used to prepare liposome and other micelles as drug delivery systems. In addition to mPEG-cholesterol, reactive or dye labeled PEG-cholesterol reagents may be offered through custom synthesis with a short turnaround. The list may include: Amine-PEG-Cholesterol, Maleimide-PEG-Cholesterol, FITC-PEG-Cholesterol, Biotin-PEG-Cholesterol, and NHS-PEG-Cholesterol etc. Cholesterol is attached to PEG through a stable amide bond.
Molecular weight: MW of PEG was measured by MALDI-MS or GPC. PDI (polydispersity index) of our linear PEG is 1.02-1.05 with very narrow MW distribution. The number of repeating ethylene oxide units (CH2CH2O) or the degree of polymerization is calculated dividing the PEG MW by 44 (44 is the molecular mass of one repeating unit).
Solubility: Soluble in water and aqueous buffer, chloroform, methylene chloride, DMF, DMSO, and less soluble in alcohol, toluene. Not soluble in ether.
Density: PEG density is approximately 1.125 g/mL
Physical form: PEG products generally appear as white or off-white powder, and for very low MW linear PEG such as MW 1k or less, it may appear as wax-like, semi-solid material due to the low MW and the type of functional groups.
Storage condition: PEG product shall be stored in the original form as received in a freezer at -20C or lower for long term storage. Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. Stock solution should avoid repeated freeze-and-thaw cycles. See Documents section for detailed storage and handling conditions.
1. Ultrasound-mediated delivery enhances therapeutic efficacy of MMP sensitive liposomes, Journal of Controlled Release ,Volume 325, 2020, 121-134, doi: 10.1016/j.jconrel.2020.06.024
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