mPEG-Azide is a linear monofunctional PEG reagent with a reactive azido (N3) group. PEG-Azide is useful in PEGylation via a click chemistry reaction with alkyne or acetylene or strained cyclooctyne.
Azide-alkyne Huisgen cycloaddition is a 1,3-dipolar cycloaddition between an azide and a terminal or internal alkyne to give a 1,2,3-triazole. This reaction is termed the Copper(I)-catalyzed Azide-Alkyne Cycloaddition (CuAAC). When alkyne is replaced with strained cyclooctyne such as DBCO, the reaction is copper (Cu)-free click chemistry. By eliminating cytotoxic copper catalysts, the bioorthogonal reaction proceeds without live cell toxicity.
Molecular weight: MW of PEG was measured by MALDI-MS or GPC. PDI (polydispersity index) of our linear PEG is 1.02-1.05 with very narrow MW distribution. The number of repeating ethylene oxide units (CH2CH2O) or the degree of polymerization is calculated dividing the PEG MW by 44 (44 is the molecular mass of one repeating unit).
Solubility: Soluble in water and aqueous buffer, chloroform, methylene chloride, DMF, DMSO, and less soluble in alcohol, toluene. Not soluble in ether.
Density: PEG density is approximately 1.125 g/mL
Physical form: PEG products generally appear as white or off-white powder, and for very low MW linear PEG such as MW 1k or less, it may appear as wax-like, semi-solid material due to the low MW and the type of functional groups.
Storage condition: PEG product shall be stored in the original form as received in a freezer at -20C or lower for long term storage. Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. Stock solution should avoid repeated freeze-and-thaw cycles. See Documents section for detailed storage and handling conditions.
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6. Enhancing intracranial delivery of clinically relevant non-viral gene vectors, RSC Adv., 2016,6, 41665-41674, Text.
7. Ren, Xi, et al. “Metabolic glycan labeling and chemoselective functionalization of native biomaterials.” Biomaterials (2018), Text.
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