Description
mPEG-Alkoxyamine reagents are useful for pegylating glycoproteins and other molecules that have oxidizable polysaccharides groups. The alkoxyamine group (also called an aminooxy, aminoxy or oxyamine group) conjugates to aldehydes of oxidized sugars. mPEG-Alkoxyamine reagents contain an extended spacer arm that is a flexible, non-immunogenic hydrophilic polyethylene glycol (PEG), which imparts water solubility to labeled molecules.
Alkoxyamines (—O-NH2) react specifically with aldehyde groups in near-neutral conditions to form stable oxime linkages. The reaction is more efficient in the presence of aniline. Alternatively, alkoxyamines can be conjugated to carboxylic acids using EDC carbodiimide chemistry. Reactive aldehyde groups can be generated in glycoproteins and other polysaccharide compounds by oxidation of constituent sugar diols using sodium periodiate. Sialic acid residues are common components of protein glycosylation and are easily converted to aldehydes with 1 mM NaIO4.
Properties
Molecular weight: MW of PEG was measured by MALDI-MS or GPC. PDI (polydispersity index) of our linear PEG is 1.02-1.05 with very narrow MW distribution. The number of repeating ethylene oxide units (CH2CH2O) or the degree of polymerization is calculated dividing the PEG MW by 44 (44 is the molecular mass of one repeating unit).
Solubility: Soluble in water and aqueous buffer, chloroform, methylene chloride, DMF, DMSO, and less soluble in alcohol, toluene. Not soluble in ether.
Density: PEG density is approximately 1.125 g/mL
Physical form: PEG products generally appear as white or off-white powder, and for very low MW linear PEG such as MW 1k or less, it may appear as wax-like, semi-solid material due to the low MW and the type of functional groups.
Storage condition: PEG product shall be stored in the original form as received in a freezer at -20C or lower for long term storage. Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. Stock solution should avoid repeated freeze-and-thaw cycles. See Documents section for detailed storage and handling conditions.
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