Description
HO-PEG-Rhodamine is a linear monofunctional PEG reagent with a tetramethylrhodamine. Also called TAMRA or TMR dye, 5(6)-carboxytetramethylrhodamine. Rhodamine is a common red fluorescent dye tracer in biology for staining cells, tissues, biomarkers or nanoparticles. It has peak fluorescence emission at 572-575 nm. mPEG derivative with other rhodamine may be available via custom synthesis. The list of dyes include Texas Red, Sulforhodamine 101 and other commercially available fluorescent dyes.
Properties
Molecular weight: MW of PEG was measured by MALDI-MS or GPC. PDI (polydispersity index) of our linear PEG is 1.02-1.05 with very narrow MW distribution. The number of repeating ethylene oxide units (CH2CH2O) or the degree of polymerization is calculated dividing the PEG MW by 44 (44 is the molecular mass of one repeating unit).
Solubility: Soluble in water and aqueous buffer, chloroform, methylene chloride, DMF, DMSO, and less soluble in alcohol, toluene. Not soluble in ether.
Density: PEG density is approximately 1.125 g/mL
Physical form: PEG products generally appear as white or off-white powder, and for very low MW linear PEG such as MW 1k or less, it may appear as wax-like, semi-solid material due to the low MW and the type of functional groups.
Storage condition: PEG product shall be stored in the original form as received in a freezer at -20C or lower for long term storage. Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. Stock solution should avoid repeated freeze-and-thaw cycles. See Documents section for detailed storage and handling conditions.
References
1. Microencapsulation of Live Cells in Synthetic Polymer Capsules, ACS Omega, 2017, 2 (6), pp 2839, Text.
2. Microstructured dextran hydrogels for burst-free sustained release of PEGylated protein drugs. Biomaterials 63 (2015): 146-157. Text.
3. AWE-somes: All Water Emulsion Bodies with Permeable Shells and Selective Compartments, ACS Appl. Mater. Interfaces, 2017, 9 (29), pp 25023, Text.
4. FRET-SLiM on native autofluorescence: a fast and reliable method to study interactions between fluorescent probes and lignin in plant cell wall. Plant methods 14.1 (2018): 74. Text.
5. NPM1 exhibits structural and dynamic heterogeneity upon phase separation with the p14ARF tumor suppressor, Journal of Magnetic Resonance, 310, 2020, 106646, Text.
6. Constructing a Biomaterial to Simulate Extracellular Drug Transport in Solid Tumors, Macromolecular Biosciences, 2020, Text.
7. Biodistribution and elimination pathways of PEGylated recombinant human deoxyribonuclease I after pulmonary delivery in mice, J. Controlled Release, 2020, Text.
8. PEGylation of recombinant human deoxyribonuclease I deceases its transport across lung epithelial cells and uptake by macrophages, International J of Pharmaceuticals, 2020, Text.
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