Fmoc-NH-PEG-NH2 is a linear heterobifunctional PEG reagent with Fmoc protected amine and a free amine. It is a useful crosslinking reagent with a PEG spacer. The free amine typically in the form of TFA or HCl salt reacts with carboxyl or NHS ester. Deprotection of Fmoc in basic conditions regenerates a second free amine. Deprotection typically occurs after the first free amine reacts with another molecules.
The fluorenylmethoxycarbonyl (Fmoc) is a base-labile protecting group. Fmoc is common in organic synthesis and peptide synthesis. Deprotection method is 20% piperidine in dimethylformamide (DMF). Fmoc has an approximate half life of 6 seconds in this solution, per "Greene's Protective Groups in Organic Synthesis"; DOI: 10.1002/9780470053485.
Molecular weight: MW of PEG was measured by MALDI-MS or GPC. PDI (polydispersity index) of our linear PEG is 1.02-1.05 with very narrow MW distribution. The number of repeating ethylene oxide units (CH2CH2O) or the degree of polymerization is calculated dividing the PEG MW by 44 (44 is the molecular mass of one repeating unit).
Solubility: Soluble in water and aqueous buffer, chloroform, methylene chloride, DMF, DMSO, and less soluble in alcohol, toluene. Not soluble in ether.
Density: PEG density is approximately 1.125 g/mL
Physical form: PEG products generally appear as white or off-white powder, and for very low MW linear PEG such as MW 1k or less, it may appear as wax-like, semi-solid material due to the low MW and the type of functional groups.
Storage condition: PEG product shall be stored in the original form as received in a freezer at -20C or lower for long term storage. Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. Stock solution should avoid repeated freeze-and-thaw cycles. See Documents section for detailed storage and handling conditions.
1. Ultrasensitive Multi-Species Detection of CRISPR-Cas9 by a Portable Centrifugal Microfluidic Platform. Analytical Methods. 2018. Text.
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