Description
Biotin-PEG-SCM is a linear heterobifunctional PEG reagent with a biotin and a succinimidyl NHS ester. It is a useful crosslinking reagent with a PEG spacer. Biotin can bind to avidin and streptavidin with high specificity and affinity. SCM stands for succinimidyl carboxyl methyl ester and can react easily with primary amine such as lysine.
Properties
Molecular weight: MW of PEG was measured by MALDI-MS or GPC. PDI (polydispersity index) of our linear PEG is 1.02-1.05 with very narrow MW distribution. The number of repeating ethylene oxide units (CH2CH2O) or the degree of polymerization is calculated dividing the PEG MW by 44 (44 is the molecular mass of one repeating unit).
Solubility: Soluble in water and aqueous buffer, chloroform, methylene chloride, DMF, DMSO, and less soluble in alcohol, toluene. Not soluble in ether.
Density: PEG density is approximately 1.125 g/mL
Physical form: PEG products generally appear as white or off-white powder, and for very low MW linear PEG such as MW 1k or less, it may appear as wax-like, semi-solid material due to the low MW and the type of functional groups.
Storage condition: PEG product shall be stored in the original form as received in a freezer at -20C or lower for long term storage. Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. Stock solution should avoid repeated freeze-and-thaw cycles. See Documents section for detailed storage and handling conditions.
References
1. Biotinylation and Amine PEGylation of Chips – Multi-modal particle manipulator to enhance bead-based bioassays, Ultrasonics. 2010 Feb;50(2):235-9, Text.
2. Biotinylation of Aminated Microbeads – Flexible Acoustic Particle Manipulation Device with Integrated Optical Waveguide for Enhanced Microbead Assays, Anal Sci. 2009; 25(2):285-91, Text.
3. Biotinylation of Aminated Surface – Experimental and Numerical Studies of Tethered DNA Shear Dynamics in the Flow-Gradient Plane, Macromolecules, 2009, 42 (22), pp 9170–9182, Text.
4. Amine PEGylation of Nucleic Acid – Reductively Responsive siRNA-Conjugated Hydrogel Nanoparticles for Gene Silencing, J. Am. Chem. Soc., 2012, 134 (17), pp 7423–7430, Text.
5. REAL-Select: Full-Length Antibody Display and Library Screening by Surface Capture on Yeast Cells.” PloS one 9.12 (2014): e114887. Text.
6. The isolation of VCAM-1+ endothelial cell-derived extracellular vesicles using microfluidics, Extracell Vesicles Circ Nucleic Acids 2024;5:83-94, Link, Text.
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