Description
Azide-PEG-azide is a linear bifunctional PEG crosslinker with two reactive azido (N3) groups. It is a useful PEGylation reagent via a click chemistry reaction between azide and alkyne or acetylene.
Azide-PEG-Azide, MW 20k
Azide-PEG-azide is a linear bifunctional PEG crosslinker with two reactive azido (N3) groups. It is a useful PEGylation reagent via a click chemistry reaction between azide and alkyne or acetylene.
Molecular weight: MW of PEG was measured by MALDI-MS or GPC. PDI (polydispersity index) of our linear PEG is 1.02-1.05 with very narrow MW distribution. The number of repeating ethylene oxide units (CH2CH2O) or the degree of polymerization is calculated dividing the PEG MW by 44 (44 is the molecular mass of one repeating unit).
Solubility: Soluble in water and aqueous buffer, chloroform, methylene chloride, DMF, DMSO, and less soluble in alcohol, toluene. Not soluble in ether.
Density: PEG density is approximately 1.125 g/mL
Physical form: PEG products generally appear as white or off-white powder, and for very low MW linear PEG such as MW 1k or less, it may appear as wax-like, semi-solid material due to the low MW and the type of functional groups.
Storage condition: PEG product shall be stored in the original form as received in a freezer at -20C or lower for long term storage. Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. Stock solution should avoid repeated freeze-and-thaw cycles. See Documents section for detailed storage and handling conditions.
1. Click Chemistry and PEG-Hyaluronan Hydrogel – Age-Related Dysfunction in Mechanotransduction Impairs Differentiation of Human Mammary Epithelial Progenitors, Cell Reports 7, 1926–1939, June 26, 2014, Text.
2. Bio-orthogonal conjugation and enzymatically triggered release of proteins within multi-layered hydrogels, Acta Biomaterialia, Volume 56, 1 July 2017, Pages 80-90, Text.
3.  Engineered hydrogels increase the post-transplantation survival of encapsulated hESC-derived midbrain dopaminergic neurons, Biomaterials, Volume 136, August 2017, Pages 1-11.  Text.
4. Main-Chain Liquid Crystalline Hydrogels that Support 3D Stem Cell Culture, Biomacromolecules  2020, doi:  10.1021/acs.biomac.0c00316
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