PEG-Tyramine

PEG-Tyramine can be used to prepare enzymatically crosslinked hydrogels. Tyramine crosslinking involves tyramine, Horseradish Peroxidase (HRP), and hydrogen peroxide (H2O2), where HRP catalyzing the oxidative coupling of tyramine, forming covalent crosslinks, often creating hydrogels for tissue engineering, drug delivery, and biosensors, with HRP activating H2O2 to create reactive intermediates that link tyramine molecules together, a reaction tunable by varying HRP/H2O2 concentrations. 1) HRP Activation: HRP uses hydrogen peroxide to become a highly reactive intermediate, 2) Tyramine Oxidation: This activated HRP then oxidizes tyramine, forming tyrosyl radicals, 3) Coupling & Crosslinking: These tyrosyl radicals rapidly react with other tyramine molecules or radicals, leading to oxidative coupling and polymerization, forming stable, covalent bonds (like C-C or C-O).