8-Arm PEG-Azide, MW 20k
8-Arm PEG-Azide is a multiarmed PEG derivative with azido at each terminal of the eight arms. One hexaglycerol core connects all eight arms through ether bonds. PEG azide is for PEGylation via click chemistry reaction with alkyne or acetylene. Azide (N3) reacts with strained cyclooctyne such as DBCO in copper-free click chemistry.
Molecular weight: 8-Arm PEG MW refers to the MW of the entire PEG molecule. The MW of each arm is 1/8 of the MW indicated in the product name. MW of PEG was measured by MALDI-MS or GPC. PDI (polydispersity index) of our 8-Arm PEG is 1.02-1.06 with very narrow MW distribution. The number of repeating ethylene oxide units (CH2CH2O) or the degree of polymerization is calculated dividing the PEG MW by 44 (44 is the molecular mass of one repeating unit).
Solubility: Soluble in water and aqueous buffer, chloroform, methylene chloride, DMF, DMSO, and less soluble in alcohol, toluene. Not soluble in ether.
Density: PEG density is approximately 1.125 g/mL
Physical form: PEG products generally appear as white or off-white powder, and for certain special 8-arm PEGs, it may appear as wax-like, semi-solid material due to low MW and the type of functional groups.
Storage condition: PEG product shall be stored in the original form as received in a freezer at -20C or lower for long term storage. Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. Stock solution should avoid repeated freeze-and-thaw cycles. See Documents section for detailed storage and handling conditions.
1. Cardiomyocyte†Driven Actuation in Biohybrid Microcylinders. Advanced Materials (2015). Text.
2. Yoon, Jaewon. Reconfigurable Shapes and Assemblies of Anisotropic Particles. Diss. The University of Michigan, 2013. Text.
3. Fabrication of modular hyaluronan-PEG hydrogels to support 3D cultures of hepatocytes in a perfused liver-on-a-chip device. Biofabrication. 2018 Dec 5. Text.
4. Dynamic peptide-folding mediated biofunctionalization and modulation of hydrogels for 4D bioprinting, Biofabrication, 2020, DOI: 10.1088/1758-5090/ab9490
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