4-Arm PEG-Azide is a multi-armed PEG derivative with azido (N3) groups at each terminal of the four arms connected to one pentaerythritol core. PEG azide is useful for PEGylation via click chemistry. Azide PEG reacts with alkyne or acetylene in copper-catalyzed click chemistry. PEG Azide reacts with DBCO and other strained cyclooctyne via copper-free click reaction.
Molecular weight: 4-Arm PEG MW refers to the MW of the entire PEG molecule. The MW of each arm is 1/4 of the MW indicated in the product name. MW of PEG was measured by MALDI-MS or GPC. PDI (polydispersity index) of our 4-Arm PEG is 1.02-1.05 with very narrow MW distribution. The number of repeating ethylene oxide units (CH2CH2O) or the degree of polymerization is calculated dividing the PEG MW by 44 (44 is the molecular mass of one repeating unit).
Solubility: Soluble in water and aqueous buffer, chloroform, methylene chloride, DMF, DMSO, and less soluble in alcohol, toluene. Not soluble in ether.
Density: PEG density is approximately 1.125 g/mL.
Physical form: PEG products generally appear as white or off-white powder, and for very low MW 4-Arm PEG such as MW 2k, it may appear as wax-like, semi-solid material due to the low MW and the type of functional groups.
Storage condition: PEG product shall be stored in the original form as received in a freezer at -20C or lower for long term storage. Stock solution of PEG reagents that do not contain oxygen or moisture sensitive functional groups may be temporarily stored in a refrigerator or ambient temperature for multiple days. Stock solution should avoid repeated freeze-and-thaw cycles. See Documents section for detailed storage and handling conditions.
1. Ultralow Protein Adsorbing Coatings from Clickable PEG Nanogel Solutions: Benefits of Attachment under Salt-Induced Phase Separation Conditions and Comparison with PEG/Albumin Nanogel Coatings. Langmuir 2013, 29, 4128-4139, Text.
2. Azide-Alkyne Click PEG Hydrogel – Supramolecular polymer gels with potential model-network structure, Polym. Chem., 2014, 5, 3018-3029, Text.
3. Neural Stem/Progenitor Cell 3-D Differentiation for Repair of Central Nervous System Injuries. Diss. The University of Akron, 2015.
4. Rapid, Photomediated Healing of Hexaarylbiimidazole-Based Covalently Cross-Linked Gels, Chem. Mater., 2017, 29 (16), pp 7023-7031, Text.
5. Connectivity defects enhance chain dynamics in supramolecular polymer model-network gels, Text.
6. In situ cross-linkable hyaluronic acid hydrogels using copper free click chemistry for cartilage tissue engineering. Polymer Chemistry. 2018; 9(1):20-7. Text.
7. Phase behavior of main-chain liquid crystalline polymer networks synthesized by alkyne-azide cycloaddition chemistry, Soft Matter, 2018, Text.
8. Preparation of type tetra-PEG ion gels through Cu-free azide-alkyne cycloaddition, Polymer J., 2020, Text.
9. Tetra-gel enables superior accuracy in combined super-resolution imaging and expansion microscopy, Scientific Reports volume 11, Article number: 16944 (2021), Text.
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