Custom PEGylated Proteins

Description

PEGylated Proteins are offered through our custom PEGylation service. Our custom PEGylation starts with selecting the source of protein (either commercially procured or provided by our clients), the number of PEG for each protein molecule, the PEGylation chemistry, analytical methods and the physical form of the PEGylated protein products. Here is a list of proteins we have PEGylated for our clients: streptavidin, human serum albumin (HSA), bovine serum albumin (BSA), fibrinogen, immunoglobulin G (IgG), hemoglobin, actin, amylase, lipase, trypsin, catalase, insulin, PAPR, collagen, matrix metalloproteinase, aminopeptidase, Taq DNA polymerase, VEGF, p53, leukocyte growth factors, uricase, adamase, interferon, erythropoietin, G-CSF, adenosine deaminase, Interleukin-2, asparaginase, Phenylalanine ammonia lyase, and Hyaluronidase. Contact our technical support to discuss your protein pegylation project.

Properties

PEGylation chemistry by default will use the lysine residues of protein to react with PEG-NHS ester forming amide bonds. If you have a different chemistry needs, please specify or discuss with our tech support. Other chemistry may include using cysteine, aspartic acid, or glycan.

PEG molecular weight options include all PEG types we offer. Typically the PEG MW may be 550, 1k, 2k, 3.4k, 5k, 10k, 20k, and 30k. The standard PEG is a linear methoxy-capped PEG (aka mPEG).

The number of PEG for each protein depends primarily on your application and is limited by the number of reactive amino acids on the protein. We have developed proprietary chemistry protocols to precisely control the number of PEG per protein molecules, ranging from 1 to 80 PEG per each protein. The protein content and the total PEG content (the number of PEG and total PEG mass) are characterized with established analytical methods. We have HPLC, GPC, SDS-PAGE, colorimetric assays for proteins and PEG, enzyme activity assays, protein binding assays and other protein specific assays.

The physical form of the final product may be lyophilized powder, solid, or solution in a specified buffer solution.

Description

Properties

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