Background on Creative PEGWorks website - leading PEG supplier.

Hyaluronate Maleimide, MW 50k

Hyaluronate Maleimide, MW 50k

Catalog No: HA-332
Product Name: Hyaluronate Maleimide, MW 50k
In Stock: Yes
SKUProduct Name
Hyaluronate Maleimide, MW 50k - 500mg
Hyaluronate Maleimide, MW 50k - 100mg
Hyaluronate Maleimide, MW 50k - 1g
Interested in Bulk Orders? Please contact us here


Hyaluronate Maleimide (HA-MAL) is a HA derivative with reactive maleimide groups. MAL selectively reacts with free thiol via Michael addition to form a stable carbon sulfur bond (thiosuccinimide). HA-MAL can be used for site specific protein and peptide modification at the cysteine amino acid site. The thiol group to react with HA-Maleimide needs to be in the form of free thiol, and often a reducing reagent agent DTT and preferably TCEP (without the need to remove excessive TCEP) is used to enhance the thiol reactivity.

The maleimide groups are conjugated to HA through an amide bond. Purity: >95% powder. Standard (default) Degree of substitution (DoS): 5 mol %, unless a different DoS is indicated in the catalog number and product name. HA-MAL derivatives with lower or higher maleimide substitution ratio (e.g. 1% and 10%) may be offered through custom synthesis service. Hyaluronic acid synonyms: HA, hyaluronate, hyaluronan.


Name and Source: Hyaluronic acid (HA, Hyaluronan, Hyaluronate) is an anionic, non-sulfated glycosaminoglycan distributed widely throughout connective, epithelial, and neural tissues. CAS# 9004-61-9. Our HA products are produced via microbial fermentation. See Documents section for details.

Appearance: White, lyophilized powder, except dye-labeled HA reagents.

Solubility: Soluble in water and the solubility data varies with MW. Higher MW results in lowered solubility. Typically solubility ranges 5-50 mg/mL, that is, 0.5%-5%. Buffered solution at pH >6 enhances solubility due to deprotonation of carboxylic acid groups and formation of carboxylate salts. HA derivatives with hydrophobic groups will lower the solubility in water, and adding alcohol or DMSO to an alcohol-water or DMSO-water mixture can dramatically improve the solubility up to 200 mg/mL (20%).

Degree of Substitution (DoS): The standard DoS is 5% by default. Product variants with special DoS other than 5% is indicated in the product name and catalog number. For example, HA-301-30% means that the DoS is 30% for this particular product.

The substitution or labeling ratio is the percentage of HA disaccharide monomers that are functionalized with the functional groups. You may calculate the number of functional groups in each HA polymer chain by 1) dividing the MW of HA by the MW of one disaccharide monomer to derive the number of disaccharide monomers in HA; 2) then multiple the number of disaccharide monomers by the labeling ratio or DoS to get the number of functional groups in each HA polymer. See Documents section for details.


1. Chemical modification of hyaluronic acid by carbodiimides, Bioconjugate chemistry, 1991, 2(4):232-41. Text.

2. Hyaluronic acid based scaffolds for tissue engineering – A review, Carbohydrate Polymers 2013, 92(2):1262-1279, Text.

3. A Rapid Crosslinkable Maleimide-Modified Hyaluronic Acid and Gelatin Hydrogel Delivery System for Regenerative Applications, Gels, 2021, 7(1), 13, Text.

Click here  to view an expanded list of hundreds of publications citing Creative PEGWorks products.


 Technical Information Hyaluronic Acid Products
 SDS of Hyaluronic Maleimide

Get In Touch

If you have any questions, please submit an online inquiry.

"*" indicates required fields

Your Name*
Do you wish to receive emails for new product introduction and sale promotions?
By submitting this form, you are consenting to our privacy policy.
This field is for validation purposes and should be left unchanged.